Single-Cell Virology: On-Chip, Quantitative Characterization of the Dynamics of Virus Spread from One Single Cell to Another

Single-Cell Virology: On-Chip, Quantitative Characterization of the Dynamics of Virus Spread from One Single Cell to Another

Blog Article

Virus spread at the single-cell level is largely uncharacterized.We have designed and constructed a microfluidic device in which each nanowell contains a single, infected cell (donor) and a single, uninfected cell (recipient).Using a GFP-expressing poliovirus as our model, we observed both lytic and non-lytic spread.Donor cells supporting lytic spread established infection earlier than those supporting non-lytic spread.However, non-lytic here spread established infections in recipient cells substantially faster than lytic spread and yielded higher rates of genome replication.

While lytic spread was sensitive to the presence of capsid entry/uncoating inhibitors, kaiser copy stands non-lytic spread was not.Consistent with emerging models for non-lytic spread of enteroviruses using autophagy, reduction in LC3 levels in cells impaired non-lytic spread and elevated the fraction of virus in donor cells spreading lytically.The ability to distinguish lytic and non-lytic spread unambiguously will enable discovery of viral and host factors and host pathways used for non-lytic spread of enteroviruses and other viruses as well.